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Cell line: | K-562 |
DSMZ no.: | ACC 10 |
Species: | human (Homo sapiens) |
Cell type: | chronic myeloid leukemia in blast crisis |
Origin: | established from the pleural effusion of a 53-year-old woman with chronic myeloid leukemia (CML) in blast crisis in 1970; cells carry the BCR-ABL1 e14-a2 (b3-a2) fusion gene; this subclone (ACC 10) weakly expresses MHC class I (image); other subclones (ACC 86 and ACC 92) are MHC class I negative and might be used in in-vitro natural killer assays; cells produce hemoglobin. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
Reference(s): | 14719, 14720, 14479, 15215, 15216, 18187, 40570 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round large, single cells in suspension; image; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.1-1.0 x 106 cells/ml; split 1:3 to 1:5 every 3 days; viability may be low after thawing, but cells recover during the following 2-3 days; starting the culture with 20% FBS in 12- or 24-well-plates could be of advantage |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30-40 hours |
Harvest: | maximal density at 1.0-1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, PCR assays |
Immunology: | CD3 -, CD14 -, CD15 +, CD19 -, CD33 +, CD71 +, CD235a +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypotriploid karyotype with sharp mode - 61-68<3n>XX, -X, -3, +7, -13, -18, +3mar, del(9)(p11/13), der(14)t(14;?)(p11;?), der(17)t(17;?)(p11/13;?), der(?18)t(15;?18)(q21;?q12), del(X)(p22) - two markers appear from FISH to have arisen from Ph;
fusion gene BCR-ABL1 is co-amplified together with MIR17HG (13q31) |
Molec. Genetics: | expression of fusion gene BCR-ABL1 (e14-a2) confirmed by RT-PCR |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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