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| Cell line: | MV4-11 |
| DSMZ no.: | ACC 102 |
| Species: | human (Homo sapiens) |
| Cell type: | acute monocytic leukemia |
| Origin: | established from a 10-year-old boy with acute monocytic leukemia (AML FAB M5) at diagnosis; cells were described in the literature to carry a t(4;11); we found that MV4-11 carries an FLT3 internal tandem duplication; patented cell line |
| Reference(s): | 14703, 14920, 14869, 14493 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | This cell line may only be provided to non-commercial researchers for research purposes. The purchaser is the end customer. §10(2) of the DSMZ terms and conditions is not applicable to this cell line. A transfer to third parties is therefore completely excluded, even if they are companies associated with the end customer. All for-profit end users requesting the MV4-11 or customers which want to transfer the cell line to a third party have to contact The Wistar Institute at busdev@wistar.org (cc to hsteinman@wistar.org) to secure a license with Wistar prior to the purchase from DSMZ. |
| | DSMZ Cell Culture Data: |
| Morphology: | single, small, round cells growing in suspension; image; image; image; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 0.6 x 106 cells/ml; cells are difficult to culture and grow slowly at first; increasing the FBS content to 20% can be of advantage; cultivation in 24-well-plates appears to be better than in large culture flasks; maintain at 0.4-1.0 x 106 cells/ml but split carefully |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50 hours |
| Harvest: | saturation density at about 1.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD-, CD4+, CD5-, CD8-, CD10-, CD13-, CD14-, CD15+, CD19-, CD33+, CD34-, HLA-DR+, CD68+, cCD3-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype - 48(46-48)<2n>XY, +8, +18, +19, -21, t(4;11)(q21;q23) |
| Molec. Genetics: | expression of fusion gene KMT2A-AFF1 (MLL-AFF1; MLL-AF4) (e9-e5) confirmed by RT-PCR |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
