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| Cell line: | N18TG2 |
| DSMZ no.: | ACC 103 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | neuroblastoma |
| Origin: | 6-thioguanine resistant mutant of cell line N18 that was established from a mouse of strain A/J with neuroblastoma C1300; cells were described in the literature to form cAMP in response to prostaglandin E1 and to be aneuploid (modal chromosome number of 81) |
| Reference(s): | 14598, 14766, 14355 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round, single, slightly adherent cells, occasionally with short processes; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS + 100 µM 6-thioguanine |
| Subculture: | split culture 1:5 to 1:10 every 3-5 days (cells grow adherent, but can be detached with trypsin); seed out at 1 x 106 cells/80 cm2 |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 40 hours |
| Harvest: | about 1.5 x 107 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
