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| Cell line: | GM-7373 |
| DSMZ no.: | ACC 109 |
| Species: | bovine (Bos taurus) |
| Cell type: | endothelial aorta |
| Origin: | established from a male bovine fetus at 24-week gestation; vascular endothelium transformed with benzo(a)pyrene in vitro; cell line described as tumorigenic |
| Reference(s): | 14580 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | cells grow as adherent multilayer; image; image; image; image; image |
| Medium: | 80% MEM (with Earle's salts) + 20% h.i. FBS + non-essential amino acids + MEM vitamins |
| Subculture: | split confluent culture 1:2 to 1:3 every 3-6 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 30-60 hours |
| Harvest: | cell harvest of about 0.8 x 105 cells/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Bos taurus species. |
| Cytogenetics: | hypodiploid bovine karyotype with 15% polyploidy - 58(54-60)<2n> - centric fusion and large submetacentric marker - corresponds to published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV -, BVDV + |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
