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| Cell line: | IEC-6 |
| DSMZ no.: | ACC 111 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | normal small intestine |
| Origin: | normal rat epithelial cells from the small intestine; cells were described in the literature to synthesize fibronectin and collagen; no continuous cell line: about 10-15 doublings after thawing of the stock cells |
| Reference(s): | 14866 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, transparent, epitheloid cells growing as monolayers (polymorphy of cell forms); image; image |
| Medium: | 45% DMEM (4.5 g/L glucose) and 45% RPMI 1640 + 10% h.i. FBS + 0.1 U/ml insulin (human or rat) |
| Subculture: | split confluent cultures 1:2 to 1:6 about twice a week using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/80 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 50 hours |
| Harvest: | about 16 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Viruses: | ELISA: reverse transcriptase negative, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
