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| Cell line: | L138.8A |
| DSMZ no.: | ACC 114 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | mast cell |
| Origin: | established from bone marrow mast cells of a female BALB/c mouse in 1984 |
| Reference(s): | 14623, 14624 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing in suspension; during exponential growth cells aggregate in small clusters; image; image; image; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | cells are initially (after thawing) difficult to grow! after thawing seed at different concentrations in 24-well plates, later maintain at 0.1-1.0 x 106 cells/ml, do not exceed 1 x 106 cells/ml; split 1:4 to 1:10 about 1-2 times per week. |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30-40 hours |
| Harvest: | maximal density at about 2.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
