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| Cell line: | MCF-7 |
| DSMZ no.: | ACC 115 |
| Species: | human (Homo sapiens) |
| Cell type: | breast adenocarcinoma |
| Origin: | established from the pleural effusion of a 69-year-old woman with metastatic invasive breast adenocarcinoma (after radio- and hormone therapy) in 1970; widely used and extensively characterized cell line; described as ER-positive, but HER2neu negative; listed in the NCI cancer cell line panel and in the CCLE (ref 18232); a derivative expressing GFP is available (ACC 983);
RNA sequencing data are available (see Ref 40906 and
RNA-Seq) |
| Reference(s): | 14975, 15191, 15219, 18201, 18232, 18234, 40236, 40906 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like cells growing as monolayers; image; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS + MEM non-essential amino acids + 1 mM sodium pyruvate + 10 µg/ml human insulin |
| Subculture: | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:4 every 2-6 days using trypsin/EDTA; cells may grow slowly after thawing |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50 hours (range 30-72 hours) |
| Harvest: | about 40-50 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To make inquiries about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypotetraploid karyotype with 8% polyploidy - 86(80-87)<4n>XX, -X, -X, -1, -1, +6, +7, -9, -10, -10, -12, -13, -13, +14, -17, -17, -18, -18, -20, -20, -20, -22, +6-8mar, add(X)(q27), add(2)(q35-37)x1-2, add(5)(p15), del(6)(q25)x1-2, add(7)(p11), add(7)(p14;hsr), add(10)(q23), del(11)(q21q23), add(15)(p11), i(15q), add(16)(q24), add(19)(q13), add(19)(p13)x1-2 - corresponds to published karyotype |
| Molec. Genetics: | expression of fusion gene BCAS4-BCAS3 confirmed by RT-PCR; Western blot: ER positive; PR, AR and HER2 negative |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
