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Cell line: | SF-21 |
DSMZ no.: | ACC 119 |
Species: | insect - fall armyworm (Spodoptera frugiperda) (Spodoptera frugiperda) |
Cell type: | ovary cells |
Origin: | this cell line (full name: IPLB-SF-21-AE) was derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae (species Spodoptera frugiperda, family Noctuidae, order Lepidoptera); cells are susceptible to Baculovirus infection; see also SF-9 (DSMZ ACC 125) which was derived from SF-21 |
Reference(s): | 15043, 14967 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | 90% round cells; 10% spindle-shaped, mostly adherent, a few cells in suspension; image; image; image; image; image |
Medium: | 80-90% TC 100 medium + 10-20% h.i. FBS (or 80-90% Grace´s insect medium + 10-20% h.i. FBS supplemented with L-Glutamine, hydrolyzed lactalbumin and yeastolate) |
Subculture: | split culture 1:3 to 1:5 every 4-5 days; cells grow partly adherent, but the use of trypsin is not necessary, detach cells with rubber scraper; seed out at ca. 1 x 106 cells/25 cm2 |
Incubation: | 25-29 °C without CO2 ; temperature below 23 °C may lead to reduced growth and loss of adherence |
Doubling time: | ca. 3 days |
Harvest: | about 25 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Spodoptera frugiperda species. |
Viruses: | PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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