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| Cell line: | L-1210 |
| DSMZ no.: | ACC 123 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | lymphocytic leukemia |
| Origin: | established from the methylcholanthrene-induced tumor in a DBA strain mouse (subline 212, 8-month-old female) in 1949; the cells were propagated by heterotransplantation into DBA/2 mice; an in-vitro suspension culture was first reported in 1966; cells are used for cytotoxicity studies |
| Reference(s): | 15148, 16802, 15214 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single round, lymphoblast-like cells in suspension; image; image |
| Medium: | 90% DMEM + 10% horse serum |
| Subculture: | maintain at 0.5-1.0 x 106 cells/ml; split 1:2 to 1:4 every 1-2 days; seed out at ca. 0.5-1.0 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50 hours |
| Harvest: | maximum density at about 2.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% horse serum, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypodiploid karyotype - 39(36-42), with about 75% polyploidy - about 77% of cells carry one or more rearrangements/aberrations |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
