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| Cell line: | MONO-MAC-6 |
| DSMZ no.: | ACC 124 |
| Species: | human (Homo sapiens) |
| Cell type: | acute monocytic leukemia |
| Origin: | established from the peripheral blood of a 64-year-old man with relapsed acute monocytic leukemia (AML FAB M5) in 1985 following myeloid metaplasia; carries t(9;11)(p22;q23) leading to KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) fusion gene; more information on this cell line on the following website: "www.monocyte.eu"; sister cell line of MONO-MAC-1 (DSM ACC 252); cells are used for the detection of pyrogens. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
| Reference(s): | 14493, 15078, 17211, 17212, 17213, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Commercial entities need a permit from the original investigator (please contact DSMZ: mutz@dsmz.de). Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | single, round/multiformed cells or small clusters of cells in suspension, sometimes loosely adherent; 1-5% giant cells; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine + non-essential amino acids + 1 mM sodium pyruvate + 10 µg/ml human insulin |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml; split ratio 1:3 to 1:5; avoid lipopolysaccharide contamination which will result in cell clumping, maturation and retarded growth; cells are best started
in 12- or 24-well plates |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-60 hours |
| Harvest: | maximal density at ca. 1.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD4 -, CD13 +, CD14 +, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +; note that CD14 expression depends on cultivation conditions; CD14 positivity cannot be guaranteed; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypotetraploid karyotype with near-diploid (8%) and polyploid (17%) sidelines - 84-90<4n>XX/XXX, -Y, +6, +7, -12, -13, -13, -16, -16, +2mar, t(9;11)(p22;q23)x2, add(10)(p11)x2, add(12)(q21), del(13)(q13q14)der(13)t(13;14)(p11;q12)x2, der(17)t(13;17)(q21;p11)x2 - first cell line shown to carry t(9;11) characteristic of AML M5 |
| Molec. Genetics: | expression of fusion gene KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) confirmed by RT-PCR |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
