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| Cell line: | SF-9 |
| DSMZ no.: | ACC 125 |
| Species: | insect - fall armyworm (Spodoptera frugiperda) (Spodoptera frugiperda) |
| Cell type: | ovary cells |
| Origin: | cloned from the parent cell line, IPLB-SF-21-AE, derived from pupal ovarian tissue of the fall armyworm (species Spodoptera frugiperda, family Noctuidae, order Lepidopt
era); cells are susceptible to infection with MNPV and other Baculoviruses; cells can be used with Baculovirus expression vectors |
| Reference(s): | 14967, 15043 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | polymorphic round cells, growing mostly adherently; image; image; image; image |
| Medium: | 90% TC 100 medium + 10% h.i. FBS (or 90% Grace´s insect medium + 10% h.i. FBS supplemented with L-Glutamine, hydrolyzed lactalbumin and yeastolate) |
| Subculture: | split culture 1:2 to 1:3 every 3-5 days (cells grow adherent, but trypsin is not necessary as cells can be detached by tapping the flask or with a rubber policeman); seed out at ca. 2 x 106 cells/T75 flask; immediately after thawing, cell viability may be low |
| Incubation: | room temperature (about 20°C) without CO2 keep lid of flask closed |
| Doubling time: | ca. 50 hours |
| Harvest: | about 5-10 x 106 cells/T75 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Spodoptera frugiperda species. |
| Viruses: | PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
