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| Cell line: | CHO-DHFR- |
| DSMZ no.: | ACC 126 |
| Species: | Chinese hamster (Cricetulus griseus) |
| Cell type: | ovary cells |
| Origin: | subclone of the parental line CHO which was derived from the ovary of an adult Chinese hamster in 1957; the cells are dehydrofolate reductase negative (dhfr-; Dukx cells); cell line also termed CHO-DG44 |
| Reference(s): | 14860, 15033, 40427 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like adherent monolayer; image; image; image |
| Medium: | 90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10% h.i. FBS + 2 mM L-glutamine (in case other media, e.g. DMEM or Iscove's MDM, are used, hypoxanthine and thymidine should be added as supplements) |
| Subculture: | seed out at ca. 0.5-2.0 x 106 cells/80 cm2; split confluent cultures 1:3 or higher every 2-4 days using trypsin/EDTA |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 20-24 hours |
| Harvest: | about 10-20 x 106 cells/80 cm2 or 40-70 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Cricetulus griseus species. |
| Cytogenetics: | near diploid hamster karyotype with 20% polyploidy - 21(20-22)<2n> - distinctive CHO morphology |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
