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| Cell line: | CX-1 |
| DSMZ no.: | ACC 129 |
| Species: | human (Homo sapiens) |
| Cell type: | colon adenocarcinoma (subclone of HT-29) |
| Origin: | cell line CX-1 was established from the human colon xenocraft model XC-1, which was established and propagated by inoculation of HT-29 cells into athymic mice; the HT-29 is also available from DSMZ (ACC 299) |
| Reference(s): | 14569, 15135 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | small circular cells, adherent, grow to large colonies in which the single cells are no longer discernible; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS |
| Subculture: | split confluent culture 1:3 to 1:6 every 3-5 days using trypsin/EDTA, seed out about 106 cells/80 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | about 30 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an identical STR profile as HT-29 in the reference STR database. |
| Cytogenetics: | human hypertriploid karyotype with 14% polyploidy - 72(65-74)<3n>XX, -X, +4, +7, -8, -8, -9, +11, +11, -13, +15, +17, -18, +2mar, der(4)t(1;4)(p11;p15)t(1;?) (p32-36;?), del(4)(q32), del(7)(p21), i(13q), add(15)(p11), del(17)(p12), add(21)(p11) - two small markers present - carries 3 markers similar to those present in HT-29 - rearrangements of chs 1, 7, 17 typical of colon carcinoma |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
