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| Cell line: | NALM-1 |
| DSMZ no.: | ACC 131 |
| Species: | human (Homo sapiens) |
| Cell type: | chronic myeloid leukemia in blast crisis |
| Origin: | established from the peripheral blood of a 3-year-old girl with chronic myeloid leukemia (CML) in blast crisis in 1975; cells were described in the literature to be TdT-positive and to carry the Philadelphia chromosome t(9;22) leading to BCR-ABL1 fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
| Reference(s): | 14769, 14479, 14488, 14768, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | small, single, round cells in suspension; image; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | cells are difficult to culture! Seed out at ca. 2 x 106 cells/ml; cells grow initially slowly; it might be of benefit to start culture in 24-well plates; maintain at 1.0-2.0 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 48 hours |
| Harvest: | maximal density at about 3-5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3-, CD10+, CD13-, CD19+, CD20(+), CD34-, CD37+, CD38+, CD80-, CD138-, HLA-DR+, sIgG-, sIgM+, cIgG-, cIgM+, sIgkappa-, sIglambda-, cIgkappa-, cIglambda-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypodiploid karyotype with 5% polyploidy - 45(42-47)<2n>X, -X, der(9)t(9;22)(q34;q12)x1-2, dup(13)(q21), der(22)t(9;22)(q34;q12) - identity confirmed |
| Molec. Genetics: | expression of fusion gene BCR-ABL1 e13-a2 (b2-a2) confirmed by RT-PCR |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
