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| Cell line: | A-10 |
| DSMZ no.: | ACC 132 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | smooth muscle |
| Origin: | established from the thoracic aorta of a DB1X embryonic rat in 1976; cells were described in the literature to display properties characteristic of smooth muscle cells |
| Reference(s): | 14662 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | large, epithelial-like, some fibroblast-like, adherent cells growing in monolayers; image; image; image; image; image |
| Medium: | 80% DMEM + 20% h.i. FBS |
| Subculture: | seed out at 1 x 106 cells/25 cm2; split confluent culture 1:2 to 1:3 once or twice a week using trypsin/EDTA; after thawing, cells may contain a significant amount of granulae; confluent cultures look more transparent |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 70-100 hours |
| Harvest: | about 1-2 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Cytogenetics: | rat karyotype |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
