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| Cell line: | 8305C |
| DSMZ no.: | ACC 133 |
| Species: | human (Homo sapiens) |
| Cell type: | thyroid carcinoma |
| Origin: | established from the primary tumor of a 67-year-old woman with primary thyroid carcinoma (undifferentiated carcinoma, composed of spindle, polygonal and giant cells) |
| Reference(s): | 14631 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, large spindle-shaped cells growing as monolayer; image; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out at about 0.5-1 x 106 cells/175 cm2; split confluent culture 1:5 once a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 55-60 hours |
| Harvest: | about 5-6 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypertriploid karyotype with 22% polyploidy - 74(67-76)<3n>XXX, -2, +9, -10, -12, -13, +14, +15, +15, +18, -19, +20, +4mar, 1-20 dm in most cells, der(6)t(6;7)(q22;q36q11.23), add(15)(p11)x2, del(17)(p12)x2 - subclonal rearrangements, e.g. del(2)(p16), del(2)(q22), del(3)(q22), i(4p), t(6p;7q), del(9)(p23) |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
