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Cell line: | PLB-985 (subclone of HL-60) |
DSMZ no.: | ACC 139 |
Species: | human (Homo sapiens) |
Cell type: | acute myeloid leukemia |
Origin: | this cell line is a widely used subclone of cell line HL-60 with comparable but some different properties |
Reference(s): | 14491, 15024, 14438, 40389 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single, round cells in suspension; image; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 1 x 106 cells/ml; maintain at ca. 0.5-1 x 106 cells/ml; split 1:2 to 1:5 every 2-3 days; minimum density at 0.2 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30 hours |
Harvest: | maximal density at about 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to that of cell line HL-60 |
Cytogenetics: | human near diploid karyotype with 4% polyploidy - 46(44-50)<2n>X, -X, +6, dm, dup(1)(q21q41), del(3)(p21), del(6)(q21)x2 |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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