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Cell line: | BPH-1 |
DSMZ no.: | ACC 143 |
Species: | human (Homo sapiens) |
Cell type: | benign prostate hyperplasia |
Origin: | prostate epithelial cells from a 68-year-old man with benign prostate hyperplasia; cells were immortalized with SV-40 large T-antigen; cells were described in the literature to express cytokeratins 8, 18, and 19 (but not 14) and to metabolize prostatic androgens |
Reference(s): | 14602 |
Biosafety level: | 1, GMO-S1 |
Risk assessment: | genetically modified organism, according to German and European legislation assigned to safety group S1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent epitheloid cells growing as monolayers; image; image; image; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS + 20 ng/ml testosterone + 5 µg/ml transferrin + 5 ng/ml sodium selenite + 5 µg/ml insulin |
Subculture: | seed out at 0.5-1 x 106 cells in a 80cm2 flask; split confluent culture 1:3 to 1:8 every 3-7 days using trypsin/EDTA (cells should be incubated for more than 10 min with trypsin/EDTA at 37 °C) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 50 hours |
Harvest: | cell harvest of ca. 3 x 106 cells/80cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypertriploid karyotype with 2.5% polyploidy - 75(69-79)<3n>XXY, -4, +5, +6, +7, -10, +11, -12, +15, +21, +3mar - +dmin - i(3q), add(7)(p11), i(8q), add(10)(p13) - sideline with add(13)(p12) and smaller 10p+ marker - matches published karyotype - carries large submetacentric 10p+ marker |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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