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| Cell line: | P3/NSI/1-AG4-1 |
| DSMZ no.: | ACC 145 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | myeloma |
| Origin: | P3/NSI/1-AG4-1 is a non-secreting clone of P3X63AG8 which is derived from P3K, the induced plasmacytoma MOPC-21; cells do not grow in HAT medium, extensively used in cell fusion studies; cells were described in the literature to synthesize kappa chain, but do not secrete free light chain |
| Reference(s): | 14681 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells, single and in small clusters in suspension; image; image; image; image; image |
| Medium: | 92.5-95% Iscove's MDM + 5-7.5% h.i. FBS + 2 mM L-glutamine |
| Subculture: | maintain at 0.1-0.8 x 106 cells/ml; split 1:3 to 1:10 every 1-3 days; initially after thawing, the viability may be reduced down to ca. 65%, but cells recover after about 1-2 days |
| Incubation: | at 37 °C with 7.5% CO2 |
| Doubling time: | 24 hours |
| Harvest: | maximal density at 1.2-1.4 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | hypertriploid murine karyotype with 2% polyploidy - 66(62-72)<3n> - 3-4 centric fusion markers present |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
