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established from the peripheral blood of a 26-year-old man with acute myeloid leukemia (AML M4) at diagnosis of AML (following T-non-Hodgkin lymphoma and T-ALL) in 1978; carries t(6;11)(q27;q23) leading to KMT2A-AFDN (MLL-MLLT4; MLL-AF6) fusion gene; sister cell lines are ML-1 and ML-3
seed out at ca. 0.5 x106 cells/ml; after thawing, culture should first be started with 20% FBS in 24-well-plates. Possibly, cells do not grow during the first few days and the viability may decrease; then, do not dilute, but exchange medium partially carefully. In logarithmic growth phase split 1:3 to 1:6 every 2-3 days; maintain at 0.5-1.2 x 106 cells/ml
at 37 °C with 5% CO2
ca. 60 hours
maximum density at 1.0-1.5 x 106 cells/ml
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
initial contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays