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Cell line: | SK-MEL-30 |
DSMZ no.: | ACC 151 |
Species: | human (Homo sapiens) |
Cell type: | melanoma |
Origin: | derived from tumor tissue (subcutis metastasis) of a 67-year-old man with malignant melanoma in 1975 |
Reference(s): | 14420 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent, epithelial-like cells growing as monolayers; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out about 1 x 106 cells/80 cm2;
split confluent culture 1:4 to 1:10 about 2-3 times per week using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30 hours |
Harvest: | about 6-8 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypertriploid karyotype with 3.5% polyploidy - 80(69-83)<3n>XXY-Y/XXYY, +1, +1, +2, +2, +3, +7, +10, -11, +12, +17, +18, +20, -21, +2mar, inv(6)(p11;q25), der(7)t(7;14)(p22;q22), der(9)t(9;?21)(p11;q11)x2, 14p+, der(15)t(15;?)(p12;?)x2, der(16)t(16;?)(q23;?), der(18)t(18;?)(p11;?), mar: ?der(19)t(1;19)(q12;q13) - additional subclonal rearrangements of ch : der(1)t(1;?)(p12;?) were present in a few cells |
Molec. Genetics: | Genomic sequencing and/or ARMS assay show that the cell line carries the NRAS Q61K mutation (mu/wt), but no BRAF V600 mutation |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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