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| Cell line: | BF-34 |
| DSMZ no.: | ACC 156 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | hybridoma (anti-rat fiber/myosin) |
| Origin: | this hybridoma cell line was described as secreting a mouse monoclonal antibody (IgG1) that reacts with rat neonatal fibers and, to a lower extent, with all rat adult type 2 fibers in immunofluorescence; it reacts specifically with neonatal MHCs (myosin heavy chain) in Western blotting; established in 1984 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from fetal bovine muscles |
| Reference(s): | 14401 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single, round cells in suspension (some cells adhere partly, but trypsin is not necessary to detach these cells); image; image; image |
| Medium: | 90% DMEM (4.5 g/L glucose) + 10% horse serum + 2 mM L-glutamine |
| Subculture: | seed out at ca. 0.3-0.5 x 106 cells/ml; split saturated culture at 1:2 to 1:3 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30-40 hours |
| Harvest: | maximal density at about 1.5-2.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% horse serum, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | secretion of a IgG1 monoclonal antibody |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine chromosome morphology |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
