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Cell line: | THP-1 |
DSMZ no.: | ACC 16 |
Species: | human (Homo sapiens) |
Cell type: | acute monocytic leukemia |
Origin: | established from the peripheral blood of a 1-year-old boy with acute monocytic leukemia (AML) at relapse in 1978; the cells can be used for induction of differentiation studies; the cells were described in the literature to produce lysozyme and to be phagocytic; carries t(9;11)(p21;q23) leading to KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) fusion gene. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and RNA-Seq) |
Reference(s): | 14493, 15021, 15022, 18187 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round, single cells in suspension, partly in clusters; image; image; image; image |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml (20% FBS and a 12- or 24-well plate could be of advantage); maintain at 0.2-1.0 x 106 cells/ml; split 1:2 to 1:3 every 3-4 days; note that after thawing cell viability may drop to about 40%, but cells will recover within a week |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 40-50 hours |
Harvest: | saturation density at about 1.0 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with Ciprobay (ciprofloxacin), thereafter negative in DAPI, microbiological culture and repeatedly in PCR assays |
Immunology: | CD3 -, CD4 +, CD13 +, CD15 +, CD19 -, CD33 +, CD34 -, cyCD68 +, HLA-DR +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near-tetraploid karyotype - 94(88-96)<4n>XY/XXY, -Y, +1, +3, +6, +6, -8, -13, -19, -22, -22, +2mar, add(1)(p11), del(1)(q42.2), i(2q), del(6)(p21)x2-4, i(7p), der(9)t(9;11)(p22;q23)i(9)(p10)x2, der(11)t(9;11)(p22;q23)x2, add(12)(q24)x1-2, der(13)t(8;13)(p11;p12), add(?18)(q21) - carries t(9;11) associated with AML M5 |
Molec. Genetics: | expression of fusion gene KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) confirmed by RT-PCR |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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