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| Cell line: | SIMA |
| DSMZ no.: | ACC 164 |
| Species: | human (Homo sapiens) |
| Cell type: | neuroblastoma |
| Origin: | established from the adrenal tumor tissue resected after treatment from a 20-month-old boy with neuroblastoma (stage III) in 1991 |
| Reference(s): | 14733, 40405, 18232 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | cell shape is bipolar, cells grow as loosely-adherent monolayers or by forming foci; image; image
; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture: | seed out at ca. 3-5 x 106 cells/80cm2 or 0.4 x 106 cells/ml; split confluent culture 1:3 to 1:5 every 3-6 days using trypsin/EDTA or by tapping the flask; cells may grow slowly initially forming non-adherent large cell aggregates |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | highly variable about 34-100 hours |
| Harvest: | cell harvest of about 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypotetraploid karyotype with 3.5% polypoidy - 88/89<4n>XXY/XXYY, -Y, -11, -17, -17, der(1qh++)t(1;17)(p34-35;q12)x2, t(6;9)(p21;q33)x2, ?inv(12)(q21q24)x2, der(22)t(17;22)(q21;p12)x2 - ca. 50-100 NMYC-containing dmin present in each cell - t(1;17) together with NMYC amplification is restricted to neuroblastoma - matches published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
