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Cell line: | IMR-32 |
DSMZ no.: | ACC 165 |
Species: | human (Homo sapiens) |
Cell type: | neuroblastoma |
Origin: | established from an abdominal mass from an untreated neuroblastoma (supposedly N-type) of a 1-year-old boy in 1967; described to carry amplyfied MYCN; widely used and extensively characterized cell line; listed in the CCLE (ref 18232) |
Reference(s): | 15025, 14949, 18232 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | fibroblast-like adherent cells growing as monolayer; image; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS + 1x non-essential amino acids |
Subculture: | seed out at ca. 0.5-1.0 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 3-5 days (trypsin need not be used, cells can be detached by tapping the flask or pipetting) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 40-50 hours |
Harvest: | about 5-10 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype - 49(44-51)<2n>XY, +1, +6, +12, der(1)t(1qter->1p34::hsr::17q22->qter)x2, add(16)(q24) - sideline with del(3)(p14), add(7)(q35), del(9)(q34), add(19)(q13) - NB associated changes include: N-myc amplified in hsr regions and unbalanced t(1;17) - matches published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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