| | |
|---|
| Cell line: | CACO-2 |
| DSMZ no.: | ACC 169 |
| Species: | human (Homo sapiens) |
| Cell type: | colon adenocarcinoma |
| Origin: | established from the primary colon tumor (adenocarcinoma) of a 72-year-old man in 1974; cells are described to produce mucus |
| Reference(s): | 14368, 14538, 14903 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | This cell line will only be distributed to non-profit institutions. Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial adherent cells; after splitting cells start to grow in colonies; image; image; image; image; image; image |
| Medium: | 80% MEM (with Earle's salts) + 20% h.i. FBS + 1x non-essential amino acids |
| Subculture: | cells grow slowly; seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:3 to 1:6 every 5-7 days using trypsin/EDTA or, if necessary, a cell scraper; after thawing cells might start slowly to take up growth; to avoid prolonged trypsinization time (>10 min) and decreased viability, split the culture before complete confluency |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 70-80 hours |
| Harvest: | about 5-8 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated during accessioning with BM-Cyclin (tiamulin & minocycline), thereafter negative in microbiologcal culture, RNA hybridization, and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
