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| Cell line: | J-774A.1 |
| DSMZ no.: | ACC 170 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | monocytes-macrophages |
| Origin: | established from a tumor in a female BALB/c mouse in 1968; cells were described in the literature to synthesize lysozyme and interleukin-1 and to have receptors for immunoglobulin and complement |
| Reference(s): | 14876 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | relatively small cells growing adherently; adherent cells will round up after a few days in culture; image; image; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:4 about twice a week (prior to reaching confluence); a certain amount of cells detach spontaneously if allowed to exceed confluence; adherent cells may be detached with a rubber policeman or using TEN (40 mM TrisCl pH 7.5, 1 mM EDTA pH 7.5, 150 mM NaCl) (not trypsin/EDTA) for quantitative harvesting or for seeding out of cells collected from the supernatant for passaging; use always new culture flasks, otherwise cells are difficult to detach |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 35 hours |
| Harvest: | about 4.5 x 107 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypotetraploid karyotype - 78(70-81)<4n> - in most cells a centric fragment was present - mouse chromosome morphology confirmed |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
