| | |
|---|
| Cell line: | NRK-49F |
| DSMZ no.: | ACC 172 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | kidney fibroblasts |
| Origin: | cell line was cloned from a mixed culture of normal rat kidney cells; cells exhibit contact inhibition and are very sensitive; indicator cells for viral or chemical transformation; this cell line is frequently used for TGF-ß bioassays |
| Reference(s): | 14626, 14452, 14362 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | fibroblast-like cells with a fusiform shape; image; image;image |
| Medium: | 95% DMEM + 5% h.i. FBS |
| Subculture: | split culture 1:4 to 1:5 about 2-3 times a week using trypsin/EDTA when the cells have reached 3/4 of confluence; seed out at ca. 0.5-1.0 x 106 cells/80 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30-40 hours |
| Harvest: | cell harvest of about 3.5 x 106/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Cytogenetics: | near-diploid rat karyotype with 4% polyploidy - 40(37-42)<2n> |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
