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| Cell line: | HEPA 1-6 |
| DSMZ no.: | ACC 175 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | hepatoma |
| Origin: | cell line was derived from the BW7756 tumor that arose in a C57L mouse; cells were described in the literature to secrete several liver-specific products (e.g. albumin, alpha1-antitrypsin, alpha-fetoprotein, amylase); cells may grow in serum-free medium when supplemented with dexamethasone and selenium |
| Reference(s): | 14445, 14446 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, epithelial cells growing as monolayer; image; image; image; image |
| Medium: | 90% DMEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2 or 175 cm2; split confluent culture 1:4 to 1:10 every 3-6 days using trypsin/EDTA; cells grow rather quickly |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | about 30 hours |
| Harvest: | about 10-15 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypotetraploid karyotype with centric fusion and 6% polyploidy - flat-moded, 53-70<4n> |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
