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Cell line: | 2HX-2 |
DSMZ no.: | ACC 183 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | hybridoma (anti-adenovirus 2) |
Origin: | established by fusion of SP2/0-AG14 myeloma cells with spleen cells from BALB/c mice immunized with HELA cells that were infected with adenovirus 2; cells secrete an IgG monoclonal antibody reacting with an adenovirus group specific antigen; cell line is patented |
Reference(s): | 14424 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single cells growing in suspension; image; image; image |
Medium: | 90% DMEM (4.5 g/L glucose) + 10% h.i. FBS |
Subculture: | optimal split ratio of 1:2 every 2-3 days |
Incubation: | at 37 °C with 5% CO2 |
Harvest: | cell harvest of about 1-2 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Cytogenetics: | murine karyotype with centric fusion and 16% polyploidy - 66(58-74)<3n> |
Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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