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| Cell line: | D-36 |
| DSMZ no.: | ACC 184 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | mast cells |
| Origin: | established from the bone marrow of a DBA/2 mouse in 1986; cells have been described in the literature to be IL-10 sensitive |
| Reference(s): | 14932 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | growing in suspension in small clumps; image; image; image; image; image |
| Medium: | 90% Iscove's MDM + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1 ng/ml mouse IL-4 + 1 ng/ml mouse IL-3 |
| Subculture: | maintain at about 0.2-8.0 x 105 cells/ml; split ratio of 1:4 every 2-3 days; viability of cells may drop to about 20% immediately after thawing but recovers after approximately 1 week |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 25 hours |
| Harvest: | maximum density at about 1.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine near-diploid karyotype with 2% tetraploidy - 40(37-41)<2n> - centric fusion only rarely present |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
