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| Cell line: | LCL-HO |
| DSMZ no.: | ACC 185 |
| Species: | human (Homo sapiens) |
| Cell type: | B lymphoblastoid cells (from patient with melanoma) |
| Origin: | this lymphoblastoid cell line was established by EBV transformation of peripheral blood lymphocytes; cell line was submitted as autologous to cell line MEL-HO (DSM ACC 62) that was derived from a woman with primary melanoma in 1976; however, cytogenetic and DNA fingerprinting analysis showed clearly that LCL-HO and MEL-HO are not related |
| Reference(s): | not published |
| Biosafety level: | 1 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects. |
| Permissions and restrictions: | A, C |
| | DSMZ Cell Culture Data: |
| Morphology: | lymphoblastoid-like cells growing in suspension singly or in clumps; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:3 every 2-4 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | saturation density at about 2.0-2.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, cyIgG +, cy IgM -, cykappa -, cylambda +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human diploid karyotype with 2% polyploidy and near triploid sideline (4%) - 46(45-47)<2n>XX, der(12)t(12;?6)(q24.32;p21.2) - additional pseudodiploid subclones are present with other structural and numerical rearrangements |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV +, SMRV + |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
