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| Cell line: | LN-405 |
| DSMZ no.: | ACC 189 |
| Species: | human (Homo sapiens) |
| Cell type: | astrocytoma |
| Origin: | glioblastoma cells established from an astrocytoma tumor (grade IV) of a 62-year old woman in 1986; cells were described as expressing mRNA for both TGF-ß1 and G-TSF/TGF-ß2 (but only the latter polypeptide was secreted) and expressing GFAP protein |
| Reference(s): | 14395 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | fibroblastic, grows as monolayers in culture; image; image; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS |
| Subculture: | split confluent culture 1:2 to 1:3 every 4-6 days using trypsin/EDTA; cells grow rather slowly; seed out at ca. 1.5 x 106 cells/80 cm2 |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 60 hours |
| Harvest: | cell harvest of about 3-4 x 106 cells/175 cm2 or 1.5 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypertetraploid karyotype with 37% polyploidy and with 1-3 supernumerary dm present in most cells - 102-108<4n>XXXX, +1, +2, +3, +4, +5, +7, -10, +11, +13, -16, -16, -16, +19, +20, +20, +21, +22, +8mar, del(1)(q41)x2, der(1)t(1;3)(p32;p11), add(4)(p11), add(7)(p14), add(10)(p13), i(13q) |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
