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| Cell line: | EBL |
| DSMZ no.: | ACC 192 |
| Species: | bovine (Bos taurus) |
| Cell type: | embryonic lung cells |
| Origin: | established from lung of a 7-month-old bovine fetus; useful for detection of Pasteurella multocida toxine |
| Reference(s): | 14373, 14907, 15189 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | rectangular-like cells growing adherently as monolayer; image; image; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at about 1 x 106 cells/175 cm2; split confluent culture every 3-4 days using trypsin/EDTA; for the toxine test cells should be starved and split once a week |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 70 hours |
| Harvest: | cell harvest of about 15 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | originally negative in DAPI, microbiological culture, RNA hybridization assay, and recently by PCR |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Bos taurus species. |
| Cytogenetics: | apparently bovine karyotype with bimodal ploidy distribution - 42(40-42)<2n>/80(77-84)<4n> - structurally rearranged with apparent reduction of ch number by centric fusion followed by tetraploidization |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV -, BVDV + |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
