| | |
|---|
| Cell line: | COLO-678 |
| DSMZ no.: | ACC 194 |
| Species: | human (Homo sapiens) |
| Cell type: | colon carcinoma |
| Origin: | established from a metastatic lymph node of a 69-year-old man with colon carcinoma in 1984; extensively characterized and frequently used cell line; part of the CCLE (ref 18232) |
| Reference(s): | 18229, 18230, 18231, 18232 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round or spindle-shaped adherent cells growing as monolayer; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/175 cm2; split confluent culture 1:4 every 6-7 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60-80 hours |
| Harvest: | cell harvest of about 20-25 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 4% polyploidy - 57(52-58)<2n>XXY, +X, +3, +7, +8, +10, +11, +12, +15, -17, -18, +22, +4mar, add(3)(p11), amp(7)(q11.23)x2, del(12)(q21/22q24.2)x2 - trisomy 7 and 12 are associated with colon carcinoma |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
