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| Cell line: | L-929 |
| DSMZ no.: | ACC 2 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | connective tissue fibroblasts |
| Origin: | established from the normal subcutaneous areolar and adipose tissue of a male C3H/An mouse; used as target in TNF detection assays |
| Reference(s): | 15132, 16798 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | fibroblasts growing as monolayer; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at about 2.0-3.0 x 106 cells/80 cm2; split confluent cultures 1:5 to 1:10 using trypsin (do not use trypsin/EDTA); after 2-3 days monolayer will be confluent; split 2-3 times a week |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 20-24 hours |
| Harvest: | ca. 15 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypertriploid karyotype - 61-67, 12-16 centric fusion markers present |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
