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| Cell line: | COLO-824 |
| DSMZ no.: | ACC 200 |
| Species: | human (Homo sapiens) |
| Cell type: | breast carcinoma |
| Origin: | established in 1991 from the pleural fluid of a 52-year-old woman with invasive breast carcinoma;
RNA sequence data are available (see Ref 40906 and RNA-Seq) |
| Reference(s): | 40263, 40906 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | cells grow loosely adherent in monolayers, some cells grow as round cells in clumps in suspension; image; image |
| Medium: | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture: | split 1:2 to 1:3 every 2-4 days without use of trypsin/EDTA (cells are only loosely adherent); seed out at ca. 3-5 x 106 cells/80 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60-80 hours |
| Harvest: | cell harvest of about 15-20 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human highly rearranged hyperdiploid karyotype with 11% polyploidy - 51(48-52)<2n>X, +1, -2, -6, +7, -10, -13, -15, -19, -21, -22, +12mar, del(1)(p12), der(1)t(1;?)(p12;?), der(6)t(1;6)(q25;q12), der(7)t(7;?)(q32/34;?), der(8)t(8;?)(q24;?), der(12)t(12;?)(p13;?), der(15)t(15;?)(p12;?), der(17)t(17;?)(p12;?) - carries small bisatellited marker |
| Molec. Genetics: | triple-negative due to absence of ER, PR and weak HER2 expression in Western blots |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
