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Cell line: | PA-TU-8988S |
DSMZ no.: | ACC 204 |
Species: | human (Homo sapiens) |
Cell type: | pancreas adenocarcinoma |
Origin: | established in 1985 from the liver metastasis of a primary pancreatic adenocarcinoma from a 64-year-old woman; described to carry mutations in KRAS, SMAD4 and TP53; sister cell line is PA-TU-8988T, ACC 162 |
Reference(s): | 14508, 40710 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epitheloid cells growing adherently as monolayer; image; image; image; image; image |
Medium: | 90% DMEM + 5% h.i. FBS + 5% horse serum + 2 mM L-glutamine (some researcher cultivate with horse serum only) |
Subculture: | seed out at ca. 2-4 x 106 cells/80 cm2; split confluent culture ca. 1:4 - 1:8 once a week using trypsin/EDTA |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | about 50-60 hours |
Harvest: | cell harvest of about 30 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near triploid karyotype with 6% polyploidy - 71(67-77)<3n>XXX, +3, -4, +7, -8, +9, +11, +11, -15, -17, -18, -18, -19, -21, -22, +6mar, del(1)(p21), del(3)(p11p22), del(6)(q26), del(9)(p12), dup(11)(p14->pter), del(12)(p13), add (15)(p12) |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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