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| Cell line: | A-S-30D |
| DSMZ no.: | ACC 208 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | hepatoma |
| Origin: | established from the hepatoma of a 16-month-old SD-female rat in 1988 |
| Reference(s): | 15202, 14433, 15140 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | grows as single cells in suspension; image; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS |
| Subculture: | optimal split ratio of 1:2 to 1:3 twice per week |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 29 hours |
| Harvest: | cell harvest of about 0.6 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Cytogenetics: | hypodiploid rat karyotype with 12% tetraploidy - 38(35-41)<2n> - 2 large submetacentric and 1 large metacentric centric fusion markers - sideline with 30-32/60-62 present at 14% |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
