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Cell line: | B9 |
DSMZ no.: | ACC 211 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | hybridoma |
Origin: | cells are a subclone derived from B13.29 (first described as clone 9.9) by fusion of BALB/c spleen cells with SP2/0-AG14 myeloma cells; this hybridoma is a non-producer; cells are IL-6-dependent |
Reference(s): | 14342 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round cells growing in suspension; image; image; image |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS + 50 µM mercaptoethanol + 50-100 pg/ml human IL-6 |
Subculture: | seed out at ca. 0.3-0.5 x 106 cells/ml, at best in a 24-well plate; split culture 1:2 to 1:6 every 2-3 days; add IL-6 every 1-2 days; cells have a high metabolic activity aciditying the culture medium. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 20-30 hours |
Harvest: | maximal density at about 0.8 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | bioassay for IL-6 (low cell densities are essential for reliable assays; try doubling cell dilutions beginning with cell densities of 2.5 x 104 cells/ml; duration of assay is commonly 2-3 days) |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Cytogenetics: | apparently murine chromosome morphology with extensive centric fusions |
Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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