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| Cell line: | A4-840 |
| DSMZ no.: | ACC 213 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | hybridoma (anti-fiber/myosin) |
| Origin: | described as secreting a mouse monoclonal antibody (IgM) reacting with slow muscle fibers in rodent, chicken and human and with slow myosin heavy chain on Western blots; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin |
| Reference(s): | 14620 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single round cells, lightly adherent; image; image; image |
| Medium: | 90% DMEM (4.5 g/L glucose) + 10% h.i. FBS + 2 mM L-glutamine + 1 mM sodium pyruvate + 1x non-essential amino acids |
| Subculture: | split saturated culture 1:5 every 3 days; seed out at about 0.3 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 25 hours |
| Harvest: | maximal density of about 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology: | secretion of a IgM monoclonal antibody |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine karyotype |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
