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| Cell line: | HAP-T1 |
| DSMZ no.: | ACC 222 |
| Species: | Syrian hamster (golden hamster) (Mesocricetus auratus) |
| Cell type: | pancreas carcinoma |
| Origin: | established from the BHP-induced pancreatic carcinoma of a Syrian golden hamster (Mesocricetus auratus; GN strain) in 1983 |
| Reference(s): | 14913 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing in monolayer; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS |
| Subculture: | seed out at 1-3 x 106 cells/175 cm2;
split confluent culture at 1:3 to 1: 6 every 3-5 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 25-30 hours |
| Harvest: | cell harvest of about 30-40 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mesocricetus auratus species. |
| Cytogenetics: | near diploid hamster karyotype with tetraploid sideline (18%) and 18% polyploidy - 44(40-45)<2n>X/XX - paucity of homologous chromosomes indicates a high level of rearrangement - hamster markers present - resembles published karyotype |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
