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Cell line: | 7-TD-1 |
DSMZ no.: | ACC 23 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | B cell hybridoma |
Origin: | murine myeloma cells fused with C57Bl/6 spleen cells; indicator cell line for IL-6 |
Reference(s): | 15042 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round cells, growing single or clustered in suspension; image; image; image; image; image; image |
Medium: | 90% RPMI 1640 + 5% h.i. FBS + 5% ConA spleen cell supernatant (or 10 ng/ml IL-6) + 2.5 x 10-5 M mercaptoethanol + 2 mM L-glutamine |
Subculture: | maintain at 0.1-0.3 x 106 cells/ml, optimal split ratio (daily) of 1:3, maximal split ratio of 1:5; for an IL-6 bioassay, we recommend to initially use 1.25-2.5 x 104 cells/ml and 100 µl in a 96-well-plate (it is advisable to determine the optimal cell density in various growth curves) |
Incubation: | at 37 °C with 7.5% CO2 |
Doubling time: | ca. 24-25 hours |
Harvest: | maximum density of 0.5-1.0 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | bioassay for IL-6 (low cell densities are essential for reliable assays; try doubling cell dilutions beginning with cell densities of 2.5 x 104 cells/ml; duration of assay is commonly 2-3 days) |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Cytogenetics: | murine karyotype |
Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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