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| Cell line: | IGR-1 |
| DSMZ no.: | ACC 236 |
| Species: | human (Homo sapiens) |
| Cell type: | melanoma |
| Origin: | established from the metastatic tumor in a groin lymph node of a 42-year-old man with malignant melanoma (primary tumor histology: NM) |
| Reference(s): | 14363, 40558 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, epithelial-like cells growing as monolayers (dendritic branching cells until confluence, upon contact inhibition smooth and round); image; image; image |
| Medium: | 85-90% DMEM + 10-15% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells in a T75 flask; split confluent culture 1:3 to 1:5 every 3-5 days using trypsin/EDTA |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | about 50-70 hours |
| Harvest: | cell harvest of about 30 x 106 cells of a T175 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded, hypertriploid karyotype with 10% polyploidy - 71(68-75)<3n>der(X), del(X), -Y, -2, +5, -8, +9, -10, -11, +5mar, add(X)(p21.3), del(X)(q22), add(1)(p36), der(6)t(6;9)(q22.3;q21.1)x2, add(8)(p21), add(9)(p13), del(9)(p21)x2, del(9)(q11), der(11)t(7;11)(q11;p11), add(13)(p12), add(17)(p12) - loss of 9p associated with melanoma |
| Molec. Genetics: | Genomic sequencing shows that the cell line carries the BRAF V600K mutation (mu/wt) or BRAF V600E/M (mu/mu), but no NRAS Q61 mutation |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
