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| Cell line: | PB-1 |
| DSMZ no.: | ACC 241 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | pre B cells |
| Origin: | established from the bone marrow of a CBA/C57BL mouse in 1995; cells are constitutively IL-7 dependent |
| Reference(s): | 14770 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | cells are very small and grow mainly in small clusters, but also singly in suspension; image; image |
| Medium: | 85% McCoy's 5A + 15% non-heat inactivated FBS + 50 ng/ml human IL-7 + 0.4x MEM vitamins + 1x MEM non-essential amino acids + 0.5x essential amino acids + 1 mM sodium pyruvate + 0.12% sodium bicarbonate + 2 mM L-glutamine + 50 µM mercaptoethanol |
| Subculture: | cells are difficult to culture! split saturated culture 1:2 every 2-3 days; seed out at ca. 1.0 x 106 cells/ml; after thawing, cells may initially grow rather slowly for about one week; maintain at 1-3 x 106 cells/ml. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 50 hours |
| Harvest: | maximum density at ca. 3 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | IL-7 dependency confirmed by 3H-thymidine assay |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine near-diploid karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
