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| Cell line: | GAMG |
| DSMZ no.: | ACC 242 |
| Species: | human (Homo sapiens) |
| Cell type: | glioma |
| Origin: | established from the biopsy specimens from a 42-year-old woman with glioma (histologically defined as glioblastoma) |
| Reference(s): | 14352, 15027 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, large, spindle-like cells growing in monolayers; image |
| Medium: | 90% DMEM + 10% h.i. FBS + 2 mM L-glutamine + 4x MEM non-essential amino acids |
| Subculture: | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:4 to 1:6 every 4-6 days using trypsin/EDTA |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | about 40-50 hours |
| Harvest: | cell harvest of about 10 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypotriploid karyotype with 17% polyploidy - 66(61-67)<3n>XXX, -4, +5, +7, -10, -13, -19, -22, +dm x1-2, der(1)t(1;4)(q11;p11), del(3)(p14), der(10)t(3;10)(p14;q13), del(17)(p11), del(18)(p11) - additional subclonal rearrangements, add(2)(q11), add(7)(q11), i(9q) - simultaneous +7, -10, -22 and dm recurrent in gliomas |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
