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Cell line: | SAOS-2 |
DSMZ no.: | ACC 243 |
Species: | human (Homo sapiens) |
Cell type: | osteogenic sarcoma |
Origin: | established from the primary osteogenic sarcoma of an 11-year-old girl in 1973; cells secrete particulate matter containing bone inducing agents thought to comprise bone morphogenic proteins (BMP-1, -2, -3, and -6) |
Reference(s): | 15135, 14538, 14537, 15232 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent, epithelial-like cells growing in monolayers; image; image; image; image |
Medium: | 85% McCoy's 5A + 15% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2 in 10 ml medium; split confluent culture 1:2 to 1:3 every 2-3 days using trypsin/EDTA; cells secrete particulate matter consisting of protein |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 40-45 hours |
Harvest: | cell harvest of about 10 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human highly rearranged hyperdiploid karyotype with 16% polyploidy - 56(52-58)<2n>X, -X, -2, -2, +3, -6, -8, +10, -12, +14, +18, -19, +20, -21, -21, -22, +15mar, add(1)(p22), add(3)(q27)x2, add(4)(p15), add(6)(p21), add(7)(p22), add(8)(q24), add(9)(q35), add(11)(p15), add(12)(p13), add(13)(q14), add(14)(p12)x2 - 13q14 breakpoint is associated with osteosarcomas - resembles published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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