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| Cell line: | DAN-G |
| DSMZ no.: | ACC 249 |
| Species: | human (Homo sapiens) |
| Cell type: | pancreas carcinoma |
| Origin: | derived from nude mouse xenografts
initiated from a tumor cell line which was established in 1985 from a 68 year-old female patient with pancreas carcinoma |
| Reference(s): | 40406, 40407, 40710 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent epitheloid cells growing in monolayers; image; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5-1 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 2-4 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 33 hours |
| Harvest: | cell harvest of about 1-3 x 105 cells/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human irregular hypotriploid karyotype with 3.5% polyploidy - 59(52-61)<3n>der(X), -X/Y, -X/Y, -1, -2, -3, -4, -5, +6, +7, -9, -10, -13, -16, -17, -18, -20, -22, -22, +4mar, add(2)(p?16), add(4)(q25), i(5p), add(6)(p22), add(7)(p11/13), der(7)t(3;7)(q11;p11), del(7)(p13), add(8)(p11), add(9)(p21), add(12)(p11), i(13q), der(14)t(14;?22)(q32;q12), add(15)(q26), der(19)t(19;hsr)(p13;?), add(20)(q13) |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
