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| Cell line: | WEHI-164S |
| DSMZ no.: | ACC 25 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | fibrosarcoma |
| Origin: | established from a fibrosarcoma chemically induced in a BALB/c mouse; cells were described in the literature to be sensitive to human cytotoxic monocytes, to tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) |
| Reference(s): | 14897, 15077 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | fibroblast-like cells growing as monolayers; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | split confluent cultures at 1:3 to 1:6; cells are adherent, slapping of the back of the flask by hand will detach the cells; seed out at ca. 4 x 106 cells/80 cm2 in 10 ml medium |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30-50 hours |
| Harvest: | ca. 15 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypertriploid karyotype with 20% polyploidy - 60-62<3n> - supernumerary ring/acf but no centric fusion marker present in most cells |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
