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| Cell line: | IPC-298 |
| DSMZ no.: | ACC 251 |
| Species: | human (Homo sapiens) |
| Cell type: | melanoma |
| Origin: | established from the primary tumor (right cervical) of a 64-year-old woman with cutaneous melanoma |
| Reference(s): | 14364 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, spindle-like cells (a few epitheliod cells) growing as confluent monolayer; image; image |
| Medium: | 90-95% RPMI 1640 + 5-10% h.i. FBS |
| Subculture: | seed out at ca. 2-3 x 106 cells/80 cm2;
split confluent culture 1:5 to 1:10 every 4-5 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 30 hours |
| Harvest: | cell harvest of about 15 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypertriploid karyotype with 13% polyploidy - 74(74-88)<3n>XX, -X, +1, -2, +3, -4, +6, +7, +9, -11, -11, +12, -13, -14, -15, +16, +21, +21, +4mar, dmin in 34% cells, del(3)(p14)x2, del(3)(q26.3)x2, del(6)(p23), del(7)(p14), add(7)(p22;HSR), der(9)t(9;11)(p21;q11), add(12)(p13)x3, del(12)(p13), del(17)(p12)x1-2 - sidelines with additional numerical and structural ch 7 rearrangements |
| Molec. Genetics: | Genomic sequencing and/or ARMS assay show that the cell line carries the NRAS Q61L mutation (mu/wt), but no BRAF V600 mutation |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
